Elabscience has launched a self-developed PolyHRP-Streptavidin conjugate designed to improve the sensitivity of enzyme-linked immunosorbent assay (ELISA) testing for low-abundance biomarker detection.

The new signal amplification reagent uses proprietary covalent polymerisation technology to strengthen detection signals while maintaining low background interference.

According to the company, the product was developed to address a common challenge in ELISA testing where very low biomarker concentrations can make it difficult to increase signal strength without also increasing background noise.

The PolyHRP-Streptavidin conjugate is produced by first polymerising multiple highly active HRP enzymes to form a PolyHRP backbone, which is then linked to streptavidin. This structure increases the enzyme-to-protein ratio and enables cascade signal amplification to improve assay sensitivity.

Elabscience stated that the reagent was designed to support stronger detection performance, lower background interference and long-term stability for researchers and assay developers working with low-abundance targets.

Validation studies showed consistent performance across several test batches. SEC-HPLC analysis demonstrated around 90% product recovery and a peak area coefficient of variation of 2.9%, indicating strong batch consistency. Nanoparticle tracking analysis also showed a narrow particle size distribution centred at approximately 155 nm, supporting the uniformity of the polymer structure.

Internal testing showed that the reagent delivered strong signal intensity while maintaining low background signals. Accelerated stability testing at 37°C showed that more than 90% activity remained after 11 days, indicating strong stability under accelerated conditions.

The product is compatible with standard ELISA, high-sensitivity ELISA and competitive ELISA workflows.

Elabscience has launched the PolyHRP-Streptavidin conjugate under catalogue number E-ELIR-020 as part of its signal amplification product range. It is available in 50 μL trial packs, 250 μL catalogue sizes and custom volumes above 1 mL. The reagent is stored at -20°C and has a shelf life of two years.

The company also plans to introduce a ready-to-use PolyHRP-Streptavidin detection reagent for routine and high-throughput ELISA applications.

 

Source: prnasia.com/

Report Abuse