Eurogentec

PNA Base, Unlabeled 90 % Purity - 1 base

PNA Base, Unlabeled 90 % Purity - 1 base

PNA is an artificial DNA/RNA analogue with no charged backbone. The absence of charge repulsion between PNA and the DNA/RNA complementary strand confers a higher specificity and sensitivity. PNA are also known to be resistant to the enzyme degradation and stable over a wide range of pH, temperature and salt concentrations. These properties enable PNA to be particularly useful for FISH studies and miRNA inhibition.

Length: From 6 to 23 bases
Quantity: 50 nmol
Purifications: RP-HPLC

The PNA backbone is made of repeating N-(2-aminoethyl)-glycine units linked by peptide bonds. The different bases (purines and pyrimidines) are linked to the backbone by methylene carbonyl linkage.
Unlike DNA or other DNA analogs, PNAs do not contain any pentose sugar moieties or phosphate group.
PNAs are depicted like peptides from the N-terminus to the C-terminus and it corresponds to 5’ à 3’ as in DNA.

Shorter PNA can be used as probe due to higher binding property
Typical DNA probe: 20 - 25 mer, Typical PNA probe: 13-17 mer
PNAs also show greater specificity in binding to complementary DNAs.
A PNA/DNA mismatch is more destabilizing than a mismatch in a DNA/DNA duplex. A single mismatch in mixed PNA/DNA
A single mismatch in 15mer PNA/DNA à 15 °C decreased Tm
A single mismatch in 15mer DNA/DNA à 11 °C decreased Tm
PNA/DNA(RNA) hybridization reaction is known to be over 100 times faster than DNA/DNA(RNA) hybridization (hybridization time, DNA: 3 hours to overnight, PNA: 30-45 minute)