Bioanalysis of Nucleic Acids
Bioanalysis of Nucleic Acids
KYMOS has a dedicated team supporting GLP bioanalysis of nucleic acids in preclinical toxicokinetic, pharmacokinetic studies and clinical trials. We are a GLP-certified and GCP compliant multinational CRO with decades’ worth of experience working with large pharma companies, small biotech and gene therapy innovators. We are equipped with cutting-edge Real Time qPCR (RTqPCR), LC-MS/MS and Hybridization ELISA platforms for the analysis of DNA- and RNA-based biomarkers, therapeutic oligonucleotides, vaccines and specialty work with mRNA, microRNA, siRNA, aptamers and antagomiRs. We are proficient in method transfer and basic R&D for the development of nucleic acid bioanalytical methods from scratch. Our expert team can support you in the following areas:
• Real Time qPCR for biomarker quantification
• Hybridization ELISA and LC-MS/MS for therapeutic oligonucleotides quantification
• PK (pharmacokinetics) and PD (pharmacodynamic) calculations and statistics
Real Time qPCR for GLP quantification of DNA/RNA biomarkers
• Absolute and relative ΔΔCt gene expression analysis in multiple biological matrices
• RTqPCR quantification of mRNA, siRNA, microRNA as well as aptamers and antagomiR therapeutics
• Bioanalytical investigations and biodistribution of DNA/RNA-based drugs
• Biodistribution of cell therapy products
• Quantification of residual human DNA and allografts decellularization efficiency
• Pathogen analyses by qPCR detection of viral and bacterial RNA/DNA in biological matrices
• Detection and quantification of host-related residual DNA or RNA in biopharmaceuticals
• Mycoplasma qPCR assays in in cell culture, media and biopharmaceuticals
• Ultra-sensitive qPCR detection up to 1pg/ml
• Automated magnetic bead isolation and purification of nucleic acids, proteins and cells
Hybridization ELISA AND LC-MS/MS for GLP quantification of therapeutic oligonucleotides
PK and PD calculations and statistics
We have decades of experience providing pharmacokinetic, pharmacodynamic, toxicokinetic calculations and statistics with Phoenix WinNonlin® for bioanalytical data coming from preclinical and clinical studies. These services are offered individually or as a packaged clinical study and include:
PK/PD analysis is conducted using the gold standard Phoenix WinNonlin® software 8.2 to:
Bioanalysis of Nucleic Acids
KYMOS has a dedicated team supporting GLP bioanalysis of nucleic acids in preclinical toxicokinetic, pharmacokinetic studies and clinical trials. We are a GLP-certified and GCP compliant multinational CRO with decades’ worth of experience working with large pharma companies, small biotech and gene therapy innovators. We are equipped with cutting-edge Real Time qPCR (RTqPCR), LC-MS/MS and Hybridization ELISA platforms for the analysis of DNA- and RNA-based biomarkers, therapeutic oligonucleotides, vaccines and specialty work with mRNA, microRNA, siRNA, aptamers and antagomiRs. We are proficient in method transfer and basic R&D for the development of nucleic acid bioanalytical methods from scratch. Our expert team can support you in the following areas
Real Time qPCR for GLP quantification of DNA/RNA biomarkers
• Absolute and relative ΔΔCt gene expression analysis in multiple biological matrices
• RTqPCR quantification of mRNA, siRNA, microRNA as well as aptamers and antagomiR therapeutics
• Bioanalytical investigations and biodistribution of DNA/RNA-based drugs
• Biodistribution of cell therapy products
• Quantification of residual human DNA and allografts decellularization efficiency
• Pathogen analyses by qPCR detection of viral and bacterial RNA/DNA in biological matrices
• Detection and quantification of host-related residual DNA or RNA in biopharmaceuticals
• Mycoplasma qPCR assays in in cell culture, media and biopharmaceuticals
• Ultra-sensitive qPCR detection up to 1pg/ml
• Automated magnetic bead isolation and purification of nucleic acids, proteins and cells
