CloneSelect Single-Cell Printer Series
CloneSelect Single-Cell Printer Series
Microfluidics-based system for single-cell cloning using image-based cell sorting
Eyewitness proof of monoclonality
The CloneSelect® Single-Cell Printer™ Series deposits single cells gently and with high efficiency using a patented, inkjet-like disposable, one-way dispensing cartridge. Sort cells using high-res imaging in either bright field or optional fluorescence and capture 5 images per each single cell deposit. Prove monoclonality, improve your efficiency, maintain and enrich your viability, and prevent cross-contamination.
Cell isolation proof captured
Document a sequence of five images as the cell is dispensed, providing direct image evidence of monoclonality in 96- or 384-well plate formats.
Clonal outgrowth improved
Achieve up to 8X improvement in clonal outgrowth efficiency over traditional methods. Deposit single cells with high efficiency into 96- or 384-well plates. It takes as little as ~5 min to dispense into a 96-well plate.
Cells stay healthy and clean
Maintain cell viability with gentle sorting as seen with outgrowth assays, and prevent cross contamination with sterile, disposable one-way flow cartridge.
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CloneSelect Single-Cell Printer
Features
Image-based proof
Five images are captured each time a single-cell is dispensed, providing direct, image-based evidence of monoclonality that can be used in regulatory fillings.
Fluorescence imaging and sorting
Sort viable cells based on live-cell fluorescent dye, enrich cell populations for high producers with fluorescent reporter, or isolate desired fluorescent CRISPR clones.
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Microfluidics technology
A patented, silicon microfluidic chip within the sterile cartridge generates pico-liter droplets that encapsulate cells. Only high-value droplets are seeded into plates.
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Label-free imaging and sorting
Sort cells based on size and morphology from a heterogenous cell population and select to deposit from 1 up to 100 cells per well.
Dispensing a wide variety of cell types
A wide range of cell types such as CHO, HEK293, L929, t-cells and b-cells can be dispensed. Cells can range in size from 5 and 40 um.
Sterile Cartridge
Individually-wrapped, sterile cartridges were designed so that cell samples never contact the instrument. A single cartridge can be used to print several microplates.
