It is important to achieve a clean template for your Sanger sequencing reaction without losing precious samples. Our sequencing Exo-SAP-IT PCR product cleanup reagent provides fast, easy, and convenient solutions for your PCR cleanup needs. Whether you require the fastest turnaround time or high-through out, our DNA sequencing PCR cleanup reagents ensure data quality and sequencing accuracy.
Before the PCR product is eady for Sanger sequencing, you must do a PCR cleanup step. Because Sanger sequencing is a highly accurate technique for you to read DNA sequence base by base, it is very important to clean up your reaction mixtures so that those unincorporated primers and dNTPs won’t interfere with your results.
Our one-step Applied Biosystems™ ExoSAP-IT™ Express PCR Product Cleanup Reagent enables quality sequencing results in a fraction of the time.
ExoSAP-IT reagents are a proprietary mixture of exonuclease I combined with shrimp alkaline phosphatise (SAP) in a specially formulated buffer that removes excess primers and dNTPs following a PCR reaction. Exonuclease I remove residual single-stranded primers and any single-stranded DNA produced during PCR. SAP removes the remaining dNTPs from the PCR mixture that may interfere with subsequent reactions.
