DNA-Free PCR Reagents & Assays
DNA-Free PCR Reagents & Assays
Try Molzym’s contamination-free PCR/Real-Time PCR products for the sensitive detection of bacterial and fungal DNA. Available are reagents for custom assay development and complete 16S and 18S assays.
PCR and Real-Time PCR ReagentsBroad-Range 16S & 18S Assays
Polymerases, water and master mixes are manufactured free of microbial DNA and are high in amplification activity over 40 cycles. Master mixes contain all reagents for assays to be used with custom primers.Complete assays are available for the sensitive PCR/Real-Time PCR detection of parts of 16S and 18S rRNA genes of eubacteria and fungi, respectively. Amplicon sequencing primers enable their identification.
PCR and Real-Time PCR Reagents:
Our special purification technology removes traces of DNA from PCR reagents. Taq and master mixes unfold highest sensitivity and reliability of detection of microbial DNA in assays employing your own primers.
Characters and benefits:
High activity, DNA-free Taq DNA polymerases
Hot start Taq available
PCR amplification up to 40 cycles without background
Master mixes for PCR and Real-Time PCR (fluorescent dye)
Taq and master mixes are ready for probe assays
A series of kits is especially dedicated to the sensitive and specific detection of bacterial and fungal DNA in samples. All reagents are manufactured DNA-free with respect to microbial DNA contaminations. Likewise, the reagents are highly active in their amplification performance, allowing up to 40 amplification cycles without background in negative controls. DNA-free MolTaq 16S/18S and Hot MolTaq 16S/18S are the Taq DNA polymerases of choice if you experience contamination problems with other standard Taqs. The new developed Hot MolTaq 16S/18S combines the favourable characters of MolTaq 16S/18S with hot start amplification and thereby increased specificity.
Master mixes contain pre-assembled reagents necessary for PCR, including dNTPs, buffer, and Mg2+ (3mM final concentration). Mastermix 16S/18S Basic is a ready-to-go master mix – just add your validated PCR primers and probes. Mastermix 16S/18S Dye is the master mix of choice if you want to run Real-Time PCRs with your primers using an intercalating dye.
