Promega

NanoLuc Labeling System

HaloTag®-NanoLuc® Labeling for Bioluminescent Signal-Based ELISA

The NanoLuc® Labeling System is based on NanoLuc® luciferase, which is a small (19.1kDa), stable reporter enzyme that generates a bright luminescence signal. Using the NanoLuc® Labeling System, antibodies are chemically labeled with NanoLuc to be used in direct, competition and indirect immunoassays.

HaloTag® technology is used to label antibodies with NanoLuc® luciferase. HaloTag is a fusion protein that covalently binds its ligand (HaloTag® Ligand) under physiological conditions and has been used in variety of applications, including antibody labeling. Labeling is a two-step process in which amine-reactive HaloTag® Succinimidyl Ester (O4) Ligand (509Da) reacts with primary amines of lysine amino acids on the antibodies. For this reaction, antibodies should be in an amine-free buffer without any protein preservative.