QIAamp DNA Kits
QIAamp DNA Kits
For isolation of genomic, mitochondrial, bacterial, parasite or viral DNA
Features
- Rapid purification of high-quality DNA
- No organic extraction or alcohol precipitation
- Consistent, high yields
- Complete removal of contaminants and inhibitors
- Automated purification of 1–12 samples on the QIAcube Connect
Product Details
QIAamp DNA Kits provide silica-membrane-based nucleic acid purification from tissues, swabs, CSF, blood, body fluids or washed cells from urine. In addition, genomic and mitochondrial DNA can be purified from small amounts of fresh or frozen blood, tissue and dried blood spots. Mechanical homogenization is not required as the tissues are lysed enzymatically. DNA purification from 1–12 samples can be automated on the QIAcube Connect using the dedicated QIAamp DNA Mini QIAcube Kit. Purification of DNA using the QIAamp DNA Micro Kit and QIAamp DNA Mini Kit is also automatable on the QIAcube Connect.
QIAamp DNA Mini standard protocols can also be executed using the TRACKMAN Connected system, paired with PIPETMAN M Connected pipettes, both from Gilson. The TRACKMAN Connected system guides researchers through the QIAamp DNA Mini protocols while automatically adjusting the Bluetooth-enabled PIPETMAN M Connected pipette settings. Each step of the protocol execution is recorded to accelerate reporting by generating a comprehensive run report. Download more information.
Performance
DNA purified using the QIAamp DNA Micro Kit is free of proteins, nucleases and other impurities, and is suitable for use in sensitive downstream applications, such as real-time PCR (see figure " Efficient purification of DNA from small sample sizes") and laser microdissection (LMD) PCR (see figure " Laser microdissection PCR"). Purified DNA may also be used in short-tandem repeat (STR) genotyping, single-nucleotide polymorphism (SNP) genotyping or pharmacogenomic research.
The QIAamp DNA Mini Kit yields DNA sized up to 50 kb (see figure " Purification of up to 50 kb genomic DNA"). DNA of this length denatures completely and has the highest amplification efficiency. Yields of nucleic acids or DNA depend on the starting material (see table “Typical yields with the QIAamp DNA Mini Kit”). DNA purified using the QIAamp DNA Mini Kit can be used in a wide range of downstream applications, including PCR and quantitative real-time PCR, Southern blotting, SNP and STR genotyping and pharmacogenomic research.
