QIAquick PCR Purification Kit
QIAquick PCR Purification Kit
For purification of up to 10 μg PCR products, 100 bp to 10 kb
Features
- Up to 95% recovery of ready-to-use DNA
- Cleanup of DNA up to 10 kb in three easy steps
- Gel loading dye for convenient sample analysis
Product Details
The QIAquick PCR Purification Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of PCR products >100 bp. DNA of up to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 μl. An optional pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. The procedure can be fully automated on the QIAcube Connect.
For optimal results it is recommended to use this product together with QIAvac 24 Plus.
QIAquick PCR Purification standard protocols can also be executed using the TRACKMAN Connected system, paired with PIPETMAN M Connected pipettes, both from Gilson. The TRACKMAN Connected system guides researchers through the QIAquick PCR Purification protocols while automatically adjusting the Bluetooth-enabled PIPETMAN M Connected pipette settings. Each step of the protocol execution is recorded to accelerate reporting by generating a comprehensive run report. Download more information.
Performance
The QIAquick PCR Purification procedure removes primers, nucleotides, enzymes, mineral oil, salts, and other impurities from DNA samples (see figure " Complete primer removal after PCR"). Using a microcentrifuge or vacuum manifold, DNA ranging from 100 bp to 10 kb is purified.
Principle
QIAquick Kits contain a silica membrane assembly for binding of DNA in high-salt buffer and elution with low-salt buffer or water. The purification procedure removes primers, nucleotides, enzymes, mineral oil, salts, agarose, ethidium bromide, and other impurities from DNA samples (see figure " Complete primer removal after PCR"). Silica-membrane technology eliminates the problems and inconvenience associated with loose resins and slurries. Specialized binding buffers are optimized for specific applications and promote selective adsorption of DNA molecules within particular size ranges.
