HPLC Buffers
Buffers are solutions of a weak acid and its conjugate base, or a weak base and its conjugate acid. In analytical chemistry, buffers are typically used in reversed-phase high performance liquid chromatography (RP-HPLC), when the sample contains acidic or basic functional groups. Buffers mitigate the influence of hydrogen/hydronium and hydroxide ions, subsequently reducing pH fluctuation.
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