Single Molecule Counting (SMC®) Technology
Originally developed by Singulex®, Inc., Single Molecule Counting (SMC®) technology with the SMCxPRO® platform enables researchers to measure femtogram levels of biomarkers and better understand the clinical relevance from healthy to disease. Providing maximum immunoassay performance while following a workflow similar to traditional ELISA technology, SMC® technology combines a unique assay elution step and robust digital counting to achieve improved signal-to-noise ratios over traditional immunoassay technologies. The SMC® technology thus provides enhanced quantification at both low and high levels of expression on one complete system. Learn more about SMC® technology, immunoassay kits, and how you can design your own custom assay to meet your critical research needs.
DIGITAL COUNTING IMPROVES SENSITIVITY AND DYNAMIC RANGE
The SMCxPRO® instrument captures the sum of all digital events counted above the background threshold. The digital event algorithm of the SMCxPRO® instrument computes digital events across a spectrum of time series for a single standard. The digital counting of molecules thus improves the assay sensitivity and extends the assay dynamic range beyond what can be achieved with traditional methods.
VERIFICATION CRITERIA FOR SMC® IMMUNOASSAYS
A comprehensive set of criteria evaluating ultimate performance are used to qualify verified SMC® immunoassays, including:
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