BrainPhys Neuronal Medium
BrainPhys Neuronal Medium
Promote, rather than inhibit, neuronal activity and maturity in your cultured primary or human pluripotent stem cell (hPSC)-derived neurons. Based on the formulation by Bardy and Gage (Bardy et al. PNAS, 2015), BrainPhys™ serum-free neuronal basal medium is optimized to yield a higher proportion of synaptically active neurons by mimicking the central nervous system (CNS) extracellular environment.
Use BrainPhys™ Neuronal Medium (Catalog # 05790) for long-term culture of hPSC- and CNS-derived neurons and to perform functional assays, such as microelectrode array-based recordings or live-fluorescent imaging, without shocking your cells by changing media.
To ensure cell health in long-term serum-free culture, BrainPhys™ must be combined with an appropriate supplement. For your convenience, various BrainPhys™ kits are available for primary or hPSC-derived neurons. BrainPhys™ Neuronal Medium and SM1 Kit (Catalog #05792) is recommended for culture of primary neurons. BrainPhys™ Primary Neuron Kit (Catalog #05794) is recommended for plating and culture of primary neurons. BrainPhys™ Neuronal Medium N2-A & SM1 Kit (Catalog #05793) and BrainPhys™ hPSC Neuron Kit (Catalog #05795) are recommended for the differentiation and maturation of hPSC-derived neurons, in combination with lineage-specific growth factors and/or small molecules (if necessary).
SUBTYPE
Basal Media, Specialized Media
CELL TYPE
Neural Cells, PSC-Derived, Neurons, Pluripotent Stem Cells
SPECIES
Human, Mouse, Rat
APPLICATION
Cell Culture, Differentiation, Maintenance
BRAND
BrainPhys
AREA OF INTEREST
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience, Stem Cell Biology
FORMULATION
Serum-Free
Product Advantages
• More representative of the brain’s extracellular environment
• Improved neuronal function and a higher proportion of synaptically active neurons
• Perform functional assays without changing media and shocking cells
• Supports long-term culture of ES/iPS cell- and CNS-derived neurons
• Rigorous raw material screening and quality control ensure minimal lot-to-lot variability
What's Included
- BrainPhys™ Neuronal Medium and SM1 Kit (Catalog #05792)
- BrainPhys™ Neuronal Medium, 500 mL (Catalog #05790)
- NeuroCult™ SM1 Neuronal Supplement, 10 mL (Catalog #05711)
- BrainPhys™ Neuronal Medium N2-A & SM1 Kit (Catalog #05793)
- BrainPhys™ Neuronal Medium, 500 mL (Catalog #05790)
- NeuroCult™ SM1 Neuronal Supplement, 10 mL (Catalog #05711)
- N2 Supplement-A, 5 mL (Catalog #07152)
- BrainPhys™ Primary Neuron Kit (Catalog #05794)
- NeuroCult™ Neuronal Plating Medium, 100 mL (Catalog #05713)
- NeuroCult™ SM1 Neuronal Supplement, 10 mL (Catalog #05711)
- BrainPhys™ Neuronal Medium, 500 mL (Catalog #05790)
- BrainPhys™ hPSC Neuron Kit (Catalog #05795)
- BrainPhys™ Neuronal Medium, 500 mL (Catalog #05790)
- NeuroCult™ SM1 Neuronal Supplement, 10 mL (Catalog #05711)
- N2 Supplement-A, 5 mL (Catalog #07152)
- Human Recombinant BDNF, 10 µg (Catalog #78005)
- Human Recombinant GDNF, 10 µg (Catalog #78058)
