Stemcell Technologies

Stemcell Technologies

1618 Station St, Vancouver, British Columbia, V5Z1B3, Canada

MethoCult H4034 Optimum

MethoCult H4034 Optimum

MethoCult H4034 Optimum

MethoCult™ H4034 Optimum (MethoCult™ GF H4034) is a methylcellulose-based medium for the growth and enumeration of hematopoietic progenitor cells in colony-forming unit (CFU) assays of human bone marrow, mobilized peripheral blood, peripheral blood, and cord blood samples. MethoCult™ H4034 Optimum has been formulated to support optimal growth of erythroid progenitor cells (BFU-E and CFU-E), granulocyte-macrophage progenitor cells (CFU-GM, CFU-G and CFU-M), and multipotential granulocyte, erythroid, macrophage, megakaryocyte progenitor cells (CFU-GEMM). The inclusion of G-CSF in Optimum formulations improves discrimination of myeloid colony subtypes. This formulation is compatible with STEMvision™ software for automated colony counting.

CONTAINS

• Methylcellulose in Iscove's MDM

• Fetal bovine serum

• Bovine serum albumin

• 2-Mercaptoethanol

• Recombinant human stem cell factor (SCF)

• Recombinant human interleukin 3 (IL-3)

• Recombinant human erythropoietin (EPO)

• Recombinant human granulocyte colony-stimulating factor (G-CSF)

• Recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF)

• Supplements

SUBTYPE

Semi-Solid Media, Specialized Media

CELL TYPE

Hematopoietic Stem and Progenitor Cells

SPECIES

Human, Non-Human Primate

APPLICATION

Cell Culture, Colony Assay, Functional Assay

BRAND

MethoCult

AREA OF INTEREST

Drug Discovery and Toxicity Testing, Stem Cell Biology

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