Company : Logopharm

Category : Analytical Equipment

Protein complexes

Protein-protein interactions and signaling pathways

The CompleXio platform has been specifically designed for identification of protein-protein interactions in native tissues and cells. It is based on nativesolubilisation of protein complexes followed by affinity-purification with multiple antibodies and stringent controls (preferably including target knockouts), identification by high-performance mass spectrometry (LC-MS/MS) and quantitative evaluation of results.

ComplexioLytesolubilisation buffers

ComplexioLytesolubilisation buffers from Logopharm are ready-to-use detergent buffers for efficient solubilisation of membrane proteins and protein complexes. These buffers have been successfully applied in affinity purifications, native gel electrophoresis, size exclusion chromatography and (non-)denaturing FFE.ComplexioLyte testing assays include:

  • Ultracentrifugation separation and western blot analysis for determining relative solubilisation efficiency.
  • 2D gel electrophoretic separation (native PAGE/SDS-PAGE) of solubilisates revealing target complex size distribution and integrity of reference complexes.
  • Affinity purification coupled with LC-MS/MS analysis to confirm preservation of known associated partner proteins.

Other Products

Cell surface markers

Micro-affinity purification

Membrane protein tools

Logopharm strategy